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# Trastuzumab-scFv CDR3 Optimization Variants Iter0 x HER-2 (YM_0989)
## Overview
YM_0989 are Trastuzumab designs against HER2. We explored several model hypothesis: (i) Does pre-training aid predicitivity and (ii) does the featurization of the input sequences matter. To test pretraining, we refer to `mata_descriptions` with the term **warm** to include pretraining, and **cold** to start from a randomly initialized seed. For featurization, we explored **label-encoded** sequences with a one-hot-encoder of amino acid identities, versus an **ESM-featurized** embedding to represent each sequence in the PPI.
## Experimental details
We studied the efficacy of generating binders with different model hyperparameters. This dataset includes 20828 unique VHHs and 1 unique sequences. All designs are limited to the CDRs of the proteins of interest
A more extensive methods section can be found in our publication [here](https://pmc.ncbi.nlm.nih.gov/articles/PMC12296056/).
## Misc dataset details
We define the following binders:
### A-library (scFvs)
There are several terms you can filter by:
- `TrastuzumabCDR_WT_<i>`: These are WT replicates.
- `TrastuzumabCDR_label_encoded_cold`: Label encoded sequences with no pretraining
- `TrastuzumabCDR_label_encoded_warm`: Label encoded sequences with pretraining
- `TrastuzumabCDR_esm_cold`: ESM featurized sequences with no pretraining
- `TrastuzumabCDR_esm_warm`: ESM featurized sequences with pretraining
### Alpha-library
There is only 1 target of interest.
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