| # VHH72 Optimization Variants Iter0 x CoV2-RBD (YM_0549) | |
| ## Overview | |
| YM_0549 consists of VHH72 designs against SARS CoV-2 RBD. We wanted to perturb the local landscape of VHH72, so we performed mutagenesis to observe the sensitivity of VHH-72 to different RBD targets. This dataset is great to study the local sensitivity of mutations relative to parent "WT" VHH72. | |
| ## Experimental details | |
| This dataset includes 29765 unique VHHs and 8 unique RBD sequences. The alpha-library is the same as YM_1068. | |
| A more extensive methods section can be found in our publication [here](https://pmc.ncbi.nlm.nih.gov/articles/PMC12296056/). | |
| ## Misc dataset details | |
| We define the following binders: | |
| ### A-library (scFvs) | |
| There are several terms you can filter by: | |
| - `wt_<i>`: These are WT replicates. | |
| - `candidate_`: Various mutations of VHH-72 | |
| ### Alpha-library | |
| - `SARS-CoV2_RBD_(6LZG)` | |
| - `WIV1` | |
| - `LYRa11` | |
| - `MERS_RBD` | |
| - `OMICRON_BA2_TStarr_Seq&Trunc` | |
| - `SARS-COV-2_GAMMA_RBD_LLNL_Trunc` | |
| - `SARS-CoV-2_Delta_RBD_L452R_T478K` | |
| - `SARS-CoV1_RBD_(6LZG_Mimic)` | |